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Delon,- 21+, 2nd July
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Saturday, June 27, 2009
IPPT and Me
Apart from research, i also have been trying to improve my fitness for the last 2 month, thru very lazy gym session, runs and swims. Talking about swim, I cant seems to find my blackie. its a TYR float, that is hypothesize to become invisible hiding in the storeroom that my dad claim to have stored it.
To my blackie
I fell in love with you ever since my eyes caught a glimpse of you,
Even though you cost 25.30, while your cousin 11, you were worth the cost,
I miss your touch between my thighs,
I miss your slender and sleek curves,
I miss you, blackie
You gave me buoyancy,
You helped me improved my freestyle,
Where are you blackie,
Oh, come back to me pls.
All i can find is a homolog of u in another color
if really i cant find u, i would have to settle for a cheaper one, come back to rub me against my thighs.
back to the topic of IPPT, my first 2 booking, i totally forgotten about the timing, therefore i defaulted twice. The third would earn me an fine. Attempted my first NSmen IPPT last wk, passed all except SBJ, sian. but nvm means juz need to go back redo 1 station.
Went back this morning, the first attempt at SBJ, apparently i have been trying what my bro tell me to do, the tecnhique to jump, i guess i strain my abs too much during my first attempt 215, tear a weak localise weak spot on my abdominal wall. resulting in my intestine being squeeze out. Inti tally thought to be just a muscle strain, due to lack of warm up, if so, stress abit, will do, so i stretch and attempted SBJ again, the 2nd one really too much. Delta fiber all fired up, sharp acute pain follow by throbbing pain, throbbing pain not suppose to occur with skeletal muscle! That's when i conclude there is more than just abs pain. din realise it was swollen until the MO in maju asked me to show him the site of injury. He attempt to localise the exact spot when he discovered a lump, which is postulated to be my intestine, ask me to lie back n try to push it back myself, since its too painful for him to do it, i better do it myself. In the end went to Alexander Hospital to get a referral letter for a general surgeon. HOW lucky can i get. i just lack freaking 1 CM to jump, n i got hernia.
I think i am just fated for RT. hah. all my efforts in the past 2 months. nvm, at least i slimmed down abit..
Delon
Yybt and Me
Yybt is a un-characterised Bacillus sub. bacterial protein that I have been working on for the past 2 months, its exact function is unknown, but research so far has indicate that it is involved in a novel signalling pathway, particularly unique to firmicutes (gram positive bacteria). Even more interesting, it is postulated to function as a stress sensor or for in vivo survival!! Recent discovery has associate this protein to invivo pathogenesis in medically important bacteria, such as Clostridium and Stapylococcus aures!! That is why elucidating the invivo function and understanding this protein might eventually lead to a greater understanding of bacteria signalling and even pathogenesis of medically important pathogens for novel drug targets.
We learn in biochemistry that by targeting proteins that are essential for invivo function, we can significantly reduce selective pressure for antibiotic resistance and do not disrupt normal flora composition that may lead to superinfection. That is why me n my mentor Rao Feng is so excited about researching on Yybt and its homologs, it is indeed an exciting and new area to venture into.
I use to thought biochemistry was just about kinetics and mechanism, but i was so wrong. During my 2 month research, not only did i touch alot of kinetics, i was also involved in trying to probe the invivo function. This includes classic microbiology work like acid challenge, biofilm formation. Its sad not to be able to comprehend the beauty of the 3D structure, as I haven attempt some yr 3 modules such as structural n computational biology.
Hui Jie and Qing Ting was involved in PKS, another very interesting mega-synthetase, invovle in antibiotics synthesis! They are very well trained in protein expession, purification, and protein n DNA gel, it will definately prove useful to have such skills, as protein expression is essential for most labs. Has been fun to share and come up with weird mechanism and hypothesis with huijie, thanks huijie for sharing my woos, sometime i might seem frastrated, but i never meant it.
Me experience there has been nothing but inspiring, though there was alot of dissapointment at time, but its all part of research. Lawerence table had this qoute that sound somthing like "we fear what is the unknown, therefore we seek desperately for knowledge to overcome our fear" truely inspiring. I respect all the researcher in pursing to discover the truth behind the unknown. Rao Feng is a great mentor and researcher, his hypothesis and ideas comes so fast, its amazing just to keep up in pace with him, let alone anticipate what is the next experiment to be done. I can only say he is truely inspiring. I felt really reluctant to leave the research, yet yearning to take a break. Research is exciting, intriguing but its very taxing, this experience was really inspiring, and really make me wanna be one of them someday.
For memories sake, shall upload some of the lab foto, his place can be considered the most cluttered, he ask me not to learn from him in this aspect, but if it works for him, why not?
racks of reagent all around, with HPLC vial cap all over the place
to do work, have to push everything behind, no space.
many many falcon tubes of reagent, you want what its all there from buffer to distill water, from loading dye to MgCl2. I will miss that place, abit gay, and Rao Feng also. On the last day, he asked if when is my FYP, i told him 1.5 yrs later and most prob he is not around anymore, he joke and say he better not be around, or else someting is terribly wrong with his phd. So long Yybt, i will miss ur DHH domain- phophodiesterase activity, PAS domain- heme association, DDGEF domain- ATPase activity, ppGpp, pGpG, pApA, CDG, CDA, ATP, GTP, AMPNPP, RocR. I have to move on, hopefully, nxt time when i see you i would know you better. Thanks Rao Feng, for the guidance and patience with me.
Apart from research, i also have been trying to improve my fitness for the last 2 month, thru very lazy gym session, runs and swims. Talking about swim, I cant seems to find my blackie. its a TYR float, that is hypothesize to become invisible hiding in the storeroom that my dad claim to have stored it.
To my blackie
I fell in love with you ever since my eyes caught a glimpse of you,
Even though you cost 25.30, while your cousin 11, you were worth the cost,
I miss your touch between my thighs,
I miss your slender and sleek curves,
I miss you, blackie
You gave me buoyancy,
You helped me improved my freestyle,
Where are you blackie,
Oh, come back to me pls.
if really i cant find u, i would have to settle for a cheaper one, come back to rub me against my thighs.
back to the topic of IPPT, my first 2 booking, i totally forgotten about the timing, therefore i defaulted twice. The third would earn me an fine. Attempted my first NSmen IPPT last wk, passed all except SBJ, sian. but nvm means juz need to go back redo 1 station.
Went back this morning, the first attempt at SBJ, apparently i have been trying what my bro tell me to do, the tecnhique to jump, i guess i strain my abs too much during my first attempt 215, tear a weak localise weak spot on my abdominal wall. resulting in my intestine being squeeze out. Inti tally thought to be just a muscle strain, due to lack of warm up, if so, stress abit, will do, so i stretch and attempted SBJ again, the 2nd one really too much. Delta fiber all fired up, sharp acute pain follow by throbbing pain, throbbing pain not suppose to occur with skeletal muscle! That's when i conclude there is more than just abs pain. din realise it was swollen until the MO in maju asked me to show him the site of injury. He attempt to localise the exact spot when he discovered a lump, which is postulated to be my intestine, ask me to lie back n try to push it back myself, since its too painful for him to do it, i better do it myself. In the end went to Alexander Hospital to get a referral letter for a general surgeon. HOW lucky can i get. i just lack freaking 1 CM to jump, n i got hernia.
I think i am just fated for RT. hah. all my efforts in the past 2 months. nvm, at least i slimmed down abit..
Delon
For memories sake, shall upload some of the lab foto, his place can be considered the most cluttered, he ask me not to learn from him in this aspect, but if it works for him, why not?
racks of reagent all around, with HPLC vial cap all over the place
to do work, have to push everything behind, no space.