Saturday, June 27, 2009

IPPT and Me



Apart from research, i also have been trying to improve my fitness for the last 2 month, thru very lazy gym session, runs and swims. Talking about swim, I cant seems to find my blackie. its a TYR float, that is hypothesize to become invisible hiding in the storeroom that my dad claim to have stored it.

To my blackie

I fell in love with you ever since my eyes caught a glimpse of you,
Even though you cost 25.30, while your cousin 11, you were worth the cost,


I miss your touch between my thighs,
I miss your slender and sleek curves,
I miss you, blackie


You gave me buoyancy,
You helped me improved my freestyle,
Where are you blackie,
Oh, come back to me pls.

All i can find is a homolog of u in another color




if really i cant find u, i would have to settle for a cheaper one, come back to rub me against my thighs.


back to the topic of IPPT, my first 2 booking, i totally forgotten about the timing, therefore i defaulted twice. The third would earn me an fine. Attempted my first NSmen IPPT last wk, passed all except SBJ, sian. but nvm means juz need to go back redo 1 station.

Went back this morning, the first attempt at SBJ, apparently i have been trying what my bro tell me to do, the tecnhique to jump, i guess i strain my abs too much during my first attempt 215, tear a weak localise weak spot on my abdominal wall. resulting in my intestine being squeeze out. Inti tally thought to be just a muscle strain, due to lack of warm up, if so, stress abit, will do, so i stretch and attempted SBJ again, the 2nd one really too much. Delta fiber all fired up, sharp acute pain follow by throbbing pain, throbbing pain not suppose to occur with skeletal muscle! That's when i conclude there is more than just abs pain. din realise it was swollen until the MO in maju asked me to show him the site of injury. He attempt to localise the exact spot when he discovered a lump, which is postulated to be my intestine, ask me to lie back n try to push it back myself, since its too painful for him to do it, i better do it myself. In the end went to Alexander Hospital to get a referral letter for a general surgeon. HOW lucky can i get. i just lack freaking 1 CM to jump, n i got hernia.

I think i am just fated for RT. hah. all my efforts in the past 2 months. nvm, at least i slimmed down abit..

Delon








Yybt and Me






Yybt is a un-characterised Bacillus sub. bacterial protein that I have been working on for the past 2 months, its exact function is unknown, but research so far has indicate that it is involved in a novel signalling pathway, particularly unique to firmicutes (gram positive bacteria). Even more interesting, it is postulated to function as a stress sensor or for in vivo survival!! Recent discovery has associate this protein to invivo pathogenesis in medically important bacteria, such as Clostridium and Stapylococcus aures!! That is why elucidating the invivo function and understanding this protein might eventually lead to a greater understanding of bacteria signalling and even pathogenesis of medically important pathogens for novel drug targets.



We learn in biochemistry that by targeting proteins that are essential for invivo function, we can significantly reduce selective pressure for antibiotic resistance and do not disrupt normal flora composition that may lead to superinfection. That is why me n my mentor Rao Feng is so excited about researching on Yybt and its homologs, it is indeed an exciting and new area to venture into.



I use to thought biochemistry was just about kinetics and mechanism, but i was so wrong. During my 2 month research, not only did i touch alot of kinetics, i was also involved in trying to probe the invivo function. This includes classic microbiology work like acid challenge, biofilm formation. Its sad not to be able to comprehend the beauty of the 3D structure, as I haven attempt some yr 3 modules such as structural n computational biology.


Hui Jie and Qing Ting was involved in PKS, another very interesting mega-synthetase, invovle in antibiotics synthesis! They are very well trained in protein expession, purification, and protein n DNA gel, it will definately prove useful to have such skills, as protein expression is essential for most labs. Has been fun to share and come up with weird mechanism and hypothesis with huijie, thanks huijie for sharing my woos, sometime i might seem frastrated, but i never meant it.


Me experience there has been nothing but inspiring, though there was alot of dissapointment at time, but its all part of research. Lawerence table had this qoute that sound somthing like "we fear what is the unknown, therefore we seek desperately for knowledge to overcome our fear" truely inspiring. I respect all the researcher in pursing to discover the truth behind the unknown. Rao Feng is a great mentor and researcher, his hypothesis and ideas comes so fast, its amazing just to keep up in pace with him, let alone anticipate what is the next experiment to be done. I can only say he is truely inspiring. I felt really reluctant to leave the research, yet yearning to take a break. Research is exciting, intriguing but its very taxing, this experience was really inspiring, and really make me wanna be one of them someday.


For memories sake, shall upload some of the lab foto, his place can be considered the most cluttered, he ask me not to learn from him in this aspect, but if it works for him, why not?



racks of reagent all around, with HPLC vial cap all over the place



to do work, have to push everything behind, no space.


many many falcon tubes of reagent, you want what its all there from buffer to distill water, from loading dye to MgCl2.
I will miss that place, abit gay, and Rao Feng also. On the last day, he asked if when is my FYP, i told him 1.5 yrs later and most prob he is not around anymore, he joke and say he better not be around, or else someting is terribly wrong with his phd. So long Yybt, i will miss ur DHH domain- phophodiesterase activity, PAS domain- heme association, DDGEF domain- ATPase activity, ppGpp, pGpG, pApA, CDG, CDA, ATP, GTP, AMPNPP, RocR. I have to move on, hopefully, nxt time when i see you i would know you better. Thanks Rao Feng, for the guidance and patience with me.





Monday, May 18, 2009

Thermodynamic of Relationship

Thermodynamic for some, sounds like cazy science concept, but for me, its a marvelously elegant description of the relationships among all events in the universe. Through all my jogs, and also personal experience, I shall make an humble attempt to extend its concepts into human-human relationship.
We only fear what we don't understand, so if we can understand the mechanics of relationship, there is nothing to fear!!
First Law of Thermodynamic : Conversation of Energy: Energy can be neither created nor destroyedDuring breaking of a relationship or forming a new one, Effort has to be gain or lose.
To form new relationship or new bond, Effort have to be put in! Seeing her home, going for dinner and dates, buying present. Bonds or relationship cannot be created without Effort!
To maintain the relationship, consistent effort has to be put it too. The same goes for breaking a bond, or destroying a relationship, when the amount of Effort is lesser then threshold, it will break, commonly know as "i have lost the interest or motivation to maintain the relationship", "i am sick of this". "i don't see the point anymore", all this equates to the lost of Effort that is put into a relationship. Bonds or relationship cannot be created or destroyed without the gain or lost of Effort

For the time being, Let EFFORT be Eff, and the effort put into the relationship is delta Eff. When alot of effort is invested into a relationship, delta Eff is negative, when there is a lost of effort, delta Eff is positive

Therefore 1st law of thermodynamic can be seen as "Relationship cannot be created from nothing and destroyed into nothing, Energy in terms of effort have to be taken in or lost from the system" analogous to energy cannot be create nor destroyed but can be lost or gain from the system.
Second Law of Thermodynamic : The Universe tends towards Maximum disorder (Chaos)Commonly known as universe always move towards disorder.
This provides a criterion for determining whether a process is spontaneous. Something to take note is Thermodynamic only indicates if a given reaction will occur or not, it does not provide any information about the rate of reaction.

Translating into the context of relationship, it means, Relationship or bonds will be broken no matter what. 2 Person will eventually break no matter what as the system tends towards disorder or chaos. When 2 person comes together, there is definately some differences between them that is the source of chaos. Unless you are marrying yourself in the mirror, even twins, there will be difference between 2 individual. The bigger the difference, the more spontaneous the rxn will be, means the higher probability of breaking up.
Let the characteristic of each individual be Char, and the difference in characteristic be delta Char. If two individual has high degree of similarity as compared to difference, Delta Char would be positive, if two individual has high degree of difference as compared to differences Delta Char would be negative

In summary, the 2nd law states the requirement for a reaction to be spontaneous or not. In the context of relationship, breakup is inevitable or spontaneously favorable when there is more difference then similarity. And if two person comes together spontaneously it is because of their similarity. Note that, similarity or difference does not affect the RATE of breaking or forming a relationship, but it DOES tells us if the relationship can be broken or formed.

Discussion
With just this 2 law, The original equation for describing how spontaneous the relationship will proceed is quantitate by Delta G = Delta H - T Delta S. Where Delta G is Gibbs free energy, the more negative the more spontaneous the reaction, in the context of relationship, it is:
Delta Rel = Delta Eff - T Delta Char

The more negative Delta Relationship is the more likely it will form, positive delta relationship means that breaking the bond is inevitable. Breaking n forming relationship is a reversible reaction, A large positive Delta relationship for the forward rxn (forming a relationship) also means a large negative Delta relationship for the reverse rxn (breaking a relationship). Its confusing .. haha, simply means that if A and B has a high possiblity of becoming lovers/friends (forwrd rxn), A and B also has a low possibilty of breaking up (reverse rxn).

As previously mentioned, when two people are very different Delta Char would be a very big negative value, therefore Delta Rel would be a high positive term, making the relationship very hard to form or maintain. This of course can be overcome by a LARGE negative delta eff, MEANS a lot of effort have to be put in to form or maintain a relationship whereby 2 individual are VERY different. For 2 individual that are very similar in characteristic, like the same food, same hobby, same ambition, Delta Char would be a very large positive value, thus Delta Rel would be a large negative value, which means Delta Eff can be small or even positive for the relationship to be form. Simply means if two individual are highly similar, little effort is needed to maintain or form the relationship.

Therefore to have a successful relationship with someone, according to the law of thermodynamics: Delta Rel = Delta Eff - T Delta Char2 person have to be very similar in characteristic and also put in a lot of effort, such relationship will make Delta Rel extremely negative and thus the forward rxn of forming a relationship is spontaneously favorable but reverse rxn of breaking this relationship is not favorable. To add alittle more confusion, BOTH backward n forward rxn are possible, it’s the EQUILIBRIUM, means for spontaneously favorable rxn, possibility of forming a relationship is much much higher than breaking up.
Of cos human are dynamic, and are constantly changing, if the change is towards more differences between them it would be there is a higher chance of breaking up. If as time goes by, the similarity or experience they share is more n more common, it will strengthen the relationship and possibility of breaking up is decreased. This will explain why once u get into a relationship, Delta Rel is not constant. It will keep on changing with different experience n as people change the variable changes too.
*Case study 1: Mr Pei (P) and Miss Go (G). P went to aussie to study leaving G in sg, this already increases their experience n differences, making delta Char more negative. Then as time goes by, relationship got strain by the lack of effort by P & G, therefore Delta Eff is being more positive. Both factor will increase delta Rel to a positive value, thus making breaking up a downhill rxn. Of cos we haven introduce the kinetic yet, but to continue the story, P met BITCH (enzyme) that lowers the kinetic barrier thus leading to a accelerated breakup. But the happy ending is, Mr P woke up and kick the enzyme away, in addition, Mr P have to put in a HUGE amount of effort ( a very large negative delta P) to form a relationship back with G!

*THIS IS JUST A HYPOTHETICAL SITUATION, ALL CHARACTER THAT RESEMBLES REAL-LIFE EVENTS ARE FAKE AND NOT TRUE.

For now I will end here, stay tune for Kinetics of Relationship! With love pushing (electron pushing) and complicated covalent catalysis, metal-ion catalysis!






Tuesday, January 06, 2009

I blog once in a blue moon, and apparently i think the moon is blue tonight


Yes yes, finally. Exam over, holidays over. Sch started, today is 3rd day. I am losing my motivation already, I think I over-enjoyed my holiday, losed the momentum, hard to start the engine again. Tonight i practically, slacked the night away. Some changes I need to adapt fast, for example no more looking forward to meet babe after school. This made school less looking-forward-to-go. Timetable no longer requires me to wake up at 7 almost everyday, but a drag to 5.30pm almost everyday too. Frame shift adaptation is required. If thats the case, why ain I in hall? Home is no longer functional for me. Was planning to enforce some discipline to stay in lib after school till, but dinner would burn a pocket in a almost burned out pocket.

Modules this semester for me are more imtimidating, maybe because more math and chemistry are invovled, and I feel that I am at a disadvantage compared to A lvl chem, n E/F maths JC counterparts. This was further re-emphasize in last semester Biostats which i see myself B+ing it when I got a Dist in polytechnique. Clearly the Maths standard is higher, an area I find poly students to be more lacking. The same applies for Chem.

Another difference this semester is lectures are now completed within monday and tuesday compared to the previous semester where it stretch over the week. This will definately change my study plan and time-management. The last and most important difference is, modules this sem, from what I observed so far, does not follow any particular textbook closely. This make reading up more difficult as material seems to spread across many different books. This eventually discourage reading up.

Most importantly, have to be discipline. Complacency might interfer with this. If discipline = k X motivation / complacency, k is a constant, to maintain a high discipline, i have to keep my motivation up, and complacency down. Writing it down and blogging it out is a form of annoucement, and when making a such commitment, its a stronger form of reminder I give myself compared to just going it through my head.

Road ahead is not easy, but to suceed i have to stay focus. For a better future, for a better person. I must be who I must be.

Hui jie once enlighten me: "What is the most successful cell? - Cancer"
It makes me think, Cancer is successful in a sense that it is immortal, it can maximise its full potential with no control. So what makes it sucessful? First it must break away from neighbouring cell, to metasize, to elimate the space restriction it is subjected to. Next it needs to divide uncontrollably.

If we apply this to life, its pretty much the same. To be like a cancer cell, a sucessful individual, one must break away from the norm, the surrounding, any other restriction that is withholding you. The 2nd factor is uncontrolled source of motivation to do well, endless source, no matter what others critic, how many times you failed, if you are just motivated uncontrollably, one day you will be as successful as a metasized tumor. Able to adapt to anywhere they go with no restriction, endless source of motivation, achieving its maximal potential, fullfilling self actualization. Of course such person would be successful as a individual, but like a cancerous tumor, the individual will be considered as a seperate entity from the rest of the body. Such cell/individual will be rejected by the general as a outcast, but we all know, they are immortal.

I strive to be cancerous.





Monday, September 29, 2008

Dreams

Just passed my first midterm, or quiz they call it in SBS. 30% of the module. After asking some of my polymate, we agree that its hard to maintain the same mindset we had in poly here in uni. If you find this offensive or yayapapaya, i'm sorry, thats the way we were inspired to be in poly. Last time, whenever we have something we dunno, we would approach the lecturer after lecture or in their office, they would gladly ans our question. The small classroom base lecture really faciliate this approach, where almost everyone knew each other, and we were never shy about voicing our problems. Here the lectures are so big, you just dun feel motivated to ask qns. Mayb its just that i lost my interest or motivation, but i feel in uni, reading the txtbk sometimes beats asking the lecturer, this apply to only some lecturers. How i miss the gd old poly days. You can just call the lecturer up in their room and ask if they are free, so much so we tend to subconsciously memorise their extention number.

When i was studying for this midterm, the content was so heavy compared to poly, so applying the basic principle of "must noe everyting to do well" for me, i had a hard time, mayb i m just plain dumb. I think its in the mindset, i just cant ingore the fact that i miss out some parts of the textbook, or lk at past yr and not noe how to ans them. Last time, the ability to know everything in the txtbk ease our anxiety. Now, it irritates me so much i push myself too hard. Initially i was worried about it, i tot i was a freak, having so many unhealthy thoughts. But recently i found out it wasnt only me having this adaptation problem. Many of us wanted to quit sch, becoz we cant find the source of motivation in maintaining such ridiculous mindset.
You might find me a freak, a geek, a mugger or anything equivalent. Some say that what we study now is not relavent. For me i have my dreams, nobody seems to understand them. They tell me i am pushing too hard, my mentally is so wrong, I had work so hard to come here from poly, i am not going to stop right here. Sometime i think about the future and ask myself, what i want to be, i have a idea, a ambitious dream, but somehow i fear i cant accomplish it. my motivation is leaking away, i need a sign to tell me i am right on track, to renew my motivation.
Another reason is the fact that all my female counterpart from poly seems to be doing so well, you just can ingore the stress to be on par with them. Poly had made me so competitive, its hard to really let go of it. I keep telling myself, it is myself i should be comparing to not others, but its hard to change the habit.
The good thing about this is, i dun feel sad or very negative about my life. Instead i feel good, i feel renewed. Here we have to read more papers, i find it more interesting than the textbk. Mayb i haven touch those erxin tough to understand papers yet. I am learning at a pace, i nv tot i would achieve, understanding concepts that i thought would be too hard in the past. The only regret for me, is my langugues standard, so far, it has been an obstacle for me. If only i went JC? nah, if i went there, i would never have been inspired by Dr yuan or be the Delon i am today.





Friday, September 12, 2008

Hokkien Ji vulgarities from a Physiological Perspective


I have studied reproductive system 4 times, once in O lvl, another 2 in ANP1/2 in poly. Now another time in this module physiology bs201. However this is the first time, i am able to relate many hokkien vulgarities to what i am studying. this might be the impact army has on my thinking. In this post I mean no offense to either sex.

Hokkien context of Bo ji

Bo ji- No balls, meaning no courage to accomplish certain task when challenge.
E.g "Delon bo ji to ask that chio bu for her number"

pls take note if sumone castrated he is generally termed as chao ah gua, not boji, boji is used periodically only, sumtime that person who bo ji, can have his ji coming out again: wu ji and proceed to accomplish the challenge

from a physiological perspective, there is acutally such situation when the balls of a genetic male do not protrude out (note it is not due to atrophy or shrinking till the extend of unobservable testis)

Physiological Explanation of Bo ji

During embryonic development, the testes develops in he gonadal ridge means cavity in the abdominal area, UNILL the last month (8month of gestation) Normal balls that push out (descend) throught the inguinal canal into the scrotum.

This seems quite far fetch, balls develope in t pelvic area den drop into a bag-like scrotum. but its sumting liddat.

For some, this dropping does not occur, so he have balls, but just still inside , i cant imagine his saggy balless scrotum though. This condition is cryptochidism.

The testis are outside because spermatogenesis is temperature sensitive, therefore no viable sperm will be produce, and further work have to be done to correlate whether spermless equals to inability to take up challenge. This can be done by a correlation study by examing impotent man and their challange seeking motivation scale.

so instead of using boji in daily context, we can consider replace it with the more elegant and scientific form, Cryptorchidism

eg. "eh you cryptochid ixxit? dun even dare to jump down from 7th floor"
"eh you bo ji ixxit? dun even dare to jump down from 7th floor"

but if a note to point out is not to substitute cryptochid with bo ji in scientific contxt

qns: what is the term for a person with testicular disorder that is characterise by no spermatogenesis because of the incorrect anatomical location of the testis during embryonic development

Ans: Bo ji (suppose to be cryptorchidism)


Hokkien contxt of Hum Ji


E.g "Delon hum ji arh, say he wan to run 84km, then now back out"

This is very similar to bo ji, but it is more commonly used in the context of backing out, or quiting. direct translation means like the balls shrink or restract.

Physiological Explanation of Hum ji

Spermatogenesis is a temperature sensitive process in the testis to produce the sperm, when environmental temperature changes, they body regulate the position of the testis to maintain the optimum temperature. This is done by the unconscious control by spinal reflex mechanism.

when the temp is too hot, the scrotal muscle will relax thus the balls will be hanging freely. when the temp is too cold, the scrotal muscle will contract, brining the balls nearer to the body to prevent heatloss and the body is generally warmer, passing the heat to the balls.

when you hum ji, it means you are frighten or intimidated, the limbic system in the CNS will be activated to give the emotions associated with fear, this will also activate sympathetic division of autonomic nervous system, commonly known as fight or flight, during which, there will be widespread vasocontriction of arteriole, which will means the scrotum muscle will contract. bringing about the phenomenon of Hum Ji, another longer term for this is Lan Pa Ji Kiew (shrink) which explain the same phenomenon. In early days when man do not wear clothes, the shrinking of balls when he is frighten or running away is observe by hokkeins and thus the incorporation of this term into their languages. You dun see American or British describing being afaid as "shrinking balls"


OMG i spent 20 mins on this dumb post, time to get back to Female reproductive system, somehow i cant seems to visualize it :(





Wednesday, July 09, 2008

WHOA finally i update my blog.. hee


1) Bangkok

i think this tittle totally fits the feelings of any reader that actually visit this site. basically i went to Bangkok, to shop. Really, its 98.56412% shopping, quite pure, contaminated with having meals and taxi, tutu rides, the rest of my conscious time are shopping! Becoz of so much shopping, my weight din really spike even though we eat like theres no rice tml. For a summary of my bangkok trip go to www.lil-girly.blogspot.com for details and photos!


2) HP101

after returning from bangkok, the nxt big exciting thing was results! my first result after 2 yrs no NS. as usual its very exciting. juz wanna thank all those who made this happen, specifically, my lt johnwoo, sgt chaiyi, who help me get my ass on sudong when there was an opening, making it possible for me to even attend special sem :D.
3) Birthday!

Best birthday ever, surprises, gift and everything, thankz babe for making it so meaningful for me :D its way better then my 21, hee. started out quite sian in the beginning, coz we only met at 5pm. went to Ma Maison at central to have dinner. i love the hamburger steak :D. the atmosphere/ ambiance was great, just the yellow lamp was abit hot though. Pictures speaks a thousand word. so let them do the talking :)


This is a very warm yellow lamp


A very smiley happy man

A very refreshing Clam Chowder


A Perfect Hamburger
this i must elaborate more, the egg on top is heavenly, the white wasnt very hard, the yoke was semi fluid. Meat was super juicy, i would love to learn how to make these hamburger. Babe loves the mashpotatos, it has carrot aka bunny fd in it!



the egg is smooth and very tasty to the right amount so you dun get too sick of eating it. the combination with beef curry is like waffle + icecream, beenhoon + curry, ice + coke, me and babe. A Perfect Match
*gasped*



other then the food, i had a totally of 5 prezzie!!!! i was told i just had 1, den 2, then 3, den 5. i was so confused at the beginning i gave up guessing. babe really made it a pleasant surprise. :D

1st present!!! i love cakes, theres chocolate banana, caramel cheesecake and walnut!
shes all out to ruin my diet plan, but hack, once a year i get my own cake



2nd present, a very very sweet card made by her, i just love that twist.
the 3rd present was HOUSE MD season 3, due to its unreliable source, i do not want to mention its origin. i LOVE it, beening housing for days! even downloaded and completed season 4. now waiting for season 5 in sept! my head is full of medical term, though i dunno how to spell most of it, its kinda cool to be able to name some of them, sarcadosis, amylodiosis. i bet it would be more enjoyable if only i know what they mean. lol.

4th present! SHOEBAG! i like the color :D


I wanted a bag, and i saw this bag at bangkok at 120++. lucky i din get it. at singapore its 79.
its Puma volvoracecollection messenger bag :D its really really really really really really really, damn fucking nice :)


and babe convinced me she din get it for me, and when i look inside the wrapping paper, i got the SHOCK of my day :) i love it. bottom line

End of the day, 2 very very happy humans, especially the one on the righ :D


4) Stagnation

Now i raelly want a job to buy sum stuff. i need moneyyy.

5)

every morning i wake up to the sounds of pigeon, it sounds like a gd start but trust me, it gets abit irritating. initially i am kinda worried they might fly in n disiao me or shit in my room, pigeon shit are dangerous to the health! then i realise theres 2 of them always playing around outside my window. untill 1 wk ago, i found a nest on my window grill, and 4 days ago, theres 1 egg, 2 days ago another egg. and now the pigeon is 24/7 siting on the eggs.. :) i attract pigeon

1 egg 1st



then 2

i was hoping to chase the bird away when the egg hatch, and filial imprinting will take place, if i m infornt of the baby bird for another 36 hrs, they will see me as their primary caregiver! but that will mean i have to camp beside the nest for days, so i gave up :(. i still fantasize about leading a group of baby pigeon as i walk around my rm, they follow me :)






Thursday, June 12, 2008

BANGKOK HERE I COME!